A laser light with an intensity of 1 mW at 500 nm is illuminating water to...

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A laser light with an intensity of 1 mW at 500 nm is illuminating water to measure the Raman spectrum of water. What is the ratio of Rayleigh scattered light intensity to Raman scattered light intensity if we are detecting with a lens/detector combination at a 90° angle. We are adding a fluorophore with a absorption cross-section of 4 x 10-19 cm² at 500 nm and a Quantum efficiency of 0.5 into the mixture. What is the concentration of the fluorophore that will produce the same intensity as the Raman scattering of the water? Why is this number important? How can you detect the Raman spectrum of water at the presence of high concentrations of this fluorophore. Hint: The number density for water is 3.3x10²² cm-3 A laser light with an intensity of 1 mW at 500 nm is illuminating water to measure the Raman spectrum of water. What is the ratio of Rayleigh scattered light intensity to Raman scattered light intensity if we are detecting with a lens/detector combination at a 90° angle. We are adding a fluorophore with a absorption cross-section of 4 x 10-19 cm² at 500 nm and a Quantum efficiency of 0.5 into the mixture. What is the concentration of the fluorophore that will produce the same intensity as the Raman scattering of the water? Why is this number important? How can you detect the Raman spectrum of water at the presence of high concentrations of this fluorophore. Hint: The number density for water is 3.3x10²² cm-3

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Water is arguably the most important chemical compound on our planet A great deal of scientific research has gone into understanding the structure and dynamics of water as it relates to the biochemica... View the full answer