A male mouse cell line has been generated in which the gfp (green fluorescent protein) gene has been inserted onto the X chromosome under the control of a constitutively expressed promoter. Cells expressing the gfp gene exhibit bright-green fluorescence under UV light.

a. What pattern of green fluorescence do you expect to see in this cell line?

b. The cell line is modified by introducing a segment of DNA containing Xic into an autosome. How do you expect the pattern of green fluorescence to change? Why?

c. A cell of the modified cell line described in (b) exhibits green fluorescence. Which copy of Xist—the one on the X chromosome or the one on the autosome—is being expressed? On which chromosome does its expression lead to chromatin remodeling, and how?

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