A microarray that has millions of ASOs is probed ten separate times with genomic DNA probes from
Question:
A microarray that has millions of ASOs is probed ten separate times with genomic DNA probes from ten family members. The figure shows the results at one locus for the ten family members in which a recessive mutant trait is caused by a single-nucleotide polymorphism. The test results for each individual are shown in the columns numbered 1 through 10. For each temperature, the top row shows the position on the microarray with the normal ASO and the bottom row shows the position on the microarray with the mutant ASO. A filled-in circle indicates that hybridization is present. Lightly shaded boxes have less signal than darkly shaded boxes. Using this figure for reference, complete the sentences below using the words and phrases on the left. (Not all words and phrases are used.) 1 23 4 5 6 7 89 10 normal ASO 80C mutant ASO normal ASO 60C mutant ASO normal ASO 40C mutant ASO are 100% omplementa At 80C between the genomic DNA probe and either ASO regardless of genotype. mutant At 40C, individuals regardless of genotype. with the genomic DNA probe in all have a both ASOs At 60C, labeled genomic DNA fragments hybridize only to the ASO with which they heterozygous Therefore, the of each individual can be deduced from the results obtained at 60C. no hybridization Individuals 3 and 4 are homozygous for the and have the mutant trait genotype Individuals 1, 5, 6, and 8 are homozygous for the normal neither ASO Individuals 2, 7, 9, and 10 are and are carriers of hybridizes the mutant allele. Hemizvgou