A mixture of albumen and lysozyme is separated by a column chromatography with a 1 m...
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A mixture of albumen and lysozyme is separated by a column chromatography with a 1 m length, which is filled with 500 grams of resins. The resin having a 1.2 g/cm³ density and 0.5 fractional participle porosity (Ep=0.5). Assume the resin has cylindrical pores and an average pore diameter is 25 A (10-10m). Albumin peak is detected after 5,000 seconds having a width of 600 seconds and a 0.3 absorbance peak (Cmax). Lysozyme peak is detected after 5,500 seconds having a width of 800 seconds and a 0.8 absorbance peak (Cmax). Both peaks follow Gaussian distribution with standard deviations. 2.1. What were the individual velocities of albumen and lysozyme? 2.2. What is the resolution? 2.3. What is the mass fraction of albumin in the feed mixture? A mixture of albumen and lysozyme is separated by a column chromatography with a 1 m length, which is filled with 500 grams of resins. The resin having a 1.2 g/cm³ density and 0.5 fractional participle porosity (Ep=0.5). Assume the resin has cylindrical pores and an average pore diameter is 25 A (10-10m). Albumin peak is detected after 5,000 seconds having a width of 600 seconds and a 0.3 absorbance peak (Cmax). Lysozyme peak is detected after 5,500 seconds having a width of 800 seconds and a 0.8 absorbance peak (Cmax). Both peaks follow Gaussian distribution with standard deviations. 2.1. What were the individual velocities of albumen and lysozyme? 2.2. What is the resolution? 2.3. What is the mass fraction of albumin in the feed mixture?
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Final answer i The individual velocities of albumin and lysozyme are approximately 00002ms and 000018ms respectively ii The resolution between the pea... View the full answer
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Income Tax Fundamentals 2013
ISBN: 9781285586618
31st Edition
Authors: Gerald E. Whittenburg, Martha Altus Buller, Steven L Gill
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