Question: A sample containing niacin was analysed by fluorimetry. The initial sample (10cm3) was diluted to 500cm3(C) and this latter solution was used for later analysis.

A sample containing niacin was analysed by fluorimetry. The initial sample (10cm3) was diluted to 500cm3(C) and this latter solution was used for later analysis. 5cm3 of the diluted unknown was made up with known amounts of niacin standard ( 5ppm ) and made up to 50cm3 as shown in the table Find the concentration of the unknown niacin
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