a) Show how equation 1 can be simplified, when [S] is >> K M . Provide explanations/rationales for your simplification and show your work. What commonly used kinetic parameter does this simplification generate and what does this parameter describe?

b) What kinetic parameter should be used to compare different substrates for the same enzyme and why is this the best parameter to use compared to the others we discussed?

c) Explain the difference between an enzyme inhibitor that binds competitively and one that binds uncompetitively. In your answer you should include a description of what they bind to, and how they change key kinetic parameters of the enzyme.

Using Michaelis Menten kinetics it is possible to describe the reaction rate of an enzymecatalyzed reaction using equation 1 shown below v=KM+[S]kcat[E]T[S] Equation 1