A technologist is performing an assay at 250 nm. The spectrophotometer being used is equipped with a hydrogen lamp, glass cuvettes, a diffraction grating and a photomultiplier tube. When trying to set 100%T using the blank solution, no transmitted light reaches the detector. What should the technologist do?

a. change to a tungsten lamp 

b. set 0%T with the blank 

c. use quartz cuvettes 

d. use a prism to select light

Answer rating: 100% (QA)

The detailed answer for the above question is provided below The issue being faced by the technologist is no transmitted light reaching the detector w... View the full answer