Following the example from the picture below, do the same for tryptophan and aspartate, consider pk1, pkR and pk2.

 

COOH HSCH Net charge: PH H CH COOH +1 10 8 6 4 2 COOH 0 Glutamate Jpk 2.19 pk 4.25 3.22 boo 9.67 Pop -2 PH 3.0 H COOH 10-Histidine 8 6 +2 4 2E pk, - 1.82 H +1 3.2 Peptides and Proteins CH pK = 6.0 10 20 OH (equivalents) (b) FIGURE 3-12 Titration curves for (a) glutamate and (b) histidine. The pk, of the R group is designated here as pk 0 1.0 OH (equivalents) 2.0 pl- 7.59 pk 9.17 COO HN H (He 3.0 85