Question: An acid indicator may exist in two forms: acidic form (HIn) and basic form (In). Absorptivity values measured for these two forms are listed

An acid indicator may exist in two forms: acidic form (HIn) and

 

An acid indicator may exist in two forms: acidic form (HIn) and basic form (In). Absorptivity values measured for these two forms are listed below. To determine its dissociation constant, K, a solution of this indicator at 2.0x 10-5M is buffered at pH 5.00 and studied. Absorbance values at 430nm and 570nm are measured to be 0.236 and 0.073, respectively. Find out K. Hin In 430 6.30 x 10 2.06 x 104 E570 7.12 x 10 9.61 x 10

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Calculate VCLUES We absorbance ratio R using at 430nm and 570nm R n A430 0236 A ... View full answer

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