An experiment was performed to determine the effects of an inhibitor on the breakdown of glycogen by
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Question:
- An experiment was performed to determine the effects of an inhibitor on the breakdown of glycogen by an enzyme. In an accompanying experiment, the inhibitor was added to the glycogen-enzyme suspension and reacted using the same experimental conditions. The data obtained from these experiments is tabulated below.
Glycogen (mM) | Product Formed Glycogen only (mM/min) | Product Formed Glycogen and Inhibitor (mM/min) |
0.5 | 22.6 | 15.9 |
1.0 | 32.1 | 24.9 |
1.5 | 38.1 | 28.2 |
2.0 | 41.2 | 35.1 |
2.5 | 44.7 | 40.0 |
3.0 | 48.5 | 43.3 |
(6 marks)
- Draw the Michaelis-Menten and Lineweaver-Burke plots of these dat
In the attachment.
(3 marks)
- Determine the form of inhibition observed from these results, and explain your rationale for this form.
(4 marks)
- Determine the values for Km and Vmax from these results.
Related Book For
Introduction to Probability and Statistics
ISBN: 978-1133103752
14th edition
Authors: William Mendenhall, Robert Beaver, Barbara Beaver
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