Question: answer all if possible, please 3. Two spherical proteins with pl of 6.0 are applied to an agarose gel and electrophoresed in a buffer pH
3. Two spherical proteins with pl of 6.0 are applied to an agarose gel and electrophoresed in a buffer pH 8.6. One protein is 14 kDa and the second is 68 kDa. Which protein will travel toward the positive electrode fastest (that is, migrate farther away from the sample loading point? Explain how you determined this. 4. The image below represents the agarose gel. (Assume all proteins you tested were loaded into one sample well.) The point of application and the negative positive poles are indicated. Draw and Label on the figure where each of your proteins migrated. + pH 8-6 Sample Application 5. In the figure below, demonstrate how results would have been affected if the electrophoretic separated was carried out in Buffer pH 6.0. In Buffer pH 10.5? Sample Well pH 6.0 PH 10.5
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