fil Answer the following questions before you leave Talk with the instructor to make sure your...
Fantastic news! We've Found the answer you've been seeking!
Question:
Transcribed Image Text:
fil Answer the following questions before you leave Talk with the instructor to make sure your answers are correct. You will need to discuss ALL of these questions in your lab report. LB/amp Plate 1 E. coll +PGLO plasmid LB/amp/arabinose Plate 2 LB/amp Plate 3 E. col -DGLO -pul Jaamid plasmid grow ston bos episiquey gazdo LB Plate 4 VU prizor 1. Which plate should contain the most number of cells growing (assume that transformation is not 100% efficient)? Why?way as woled sidst erit al 2. Which plate should contain the least number of cells growing? Why? entah pages 3. What do the control plates 1, 3, and 4 tell you (what is the purpose for each of these plates)? Give an explanation for each plate. For example, do you expect when growth or no growth on each plate? If you observed the opposite of what you expect, what does this mean? 4. Which plate will have green cells under a UV light? Why? 69 (AMO) 5. Which plates will have cells that are NOT green under a UV light? Why? (AMC-) 6. The (-DNA) tube does not contain the pGlo DNA, so the cells will not be transformed with the plasmid. Why does it still have to go through the heat shock process the same way as the (+DNA) tube (be specific)? will be the basis for t 81 7. An organism's environment can determine its physical trait (phenotype). a) After transformation is successful, what two factors must be present in order for you to observe the green fluorescence of the transformed cells (other than the pGlo)? Explain what each factor is doing to cause green fluorescence. fil Answer the following questions before you leave Talk with the instructor to make sure your answers are correct. You will need to discuss ALL of these questions in your lab report. LB/amp Plate 1 E. coll +PGLO plasmid LB/amp/arabinose Plate 2 LB/amp Plate 3 E. col -DGLO -pul Jaamid plasmid grow ston bos episiquey gazdo LB Plate 4 VU prizor 1. Which plate should contain the most number of cells growing (assume that transformation is not 100% efficient)? Why?way as woled sidst erit al 2. Which plate should contain the least number of cells growing? Why? entah pages 3. What do the control plates 1, 3, and 4 tell you (what is the purpose for each of these plates)? Give an explanation for each plate. For example, do you expect when growth or no growth on each plate? If you observed the opposite of what you expect, what does this mean? 4. Which plate will have green cells under a UV light? Why? 69 (AMO) 5. Which plates will have cells that are NOT green under a UV light? Why? (AMC-) 6. The (-DNA) tube does not contain the pGlo DNA, so the cells will not be transformed with the plasmid. Why does it still have to go through the heat shock process the same way as the (+DNA) tube (be specific)? will be the basis for t 81 7. An organism's environment can determine its physical trait (phenotype). a) After transformation is successful, what two factors must be present in order for you to observe the green fluorescence of the transformed cells (other than the pGlo)? Explain what each factor is doing to cause green fluorescence.
Expert Answer:
Answer rating: 100% (QA)
Answer 1 Only in LB plate plate no 4 maximum no of colonies will be found because it dose not have any antibiotic or selection marker which will kill ... View the full answer
Related Book For
Financial accounting
ISBN: 978-0136108863
8th Edition
Authors: Walter T. Harrison, Charles T. Horngren, William Bill Thomas
Posted Date:
Students also viewed these biology questions
-
Answer the following questions about real GDP per capita: a. If Country A had 4 times the initial level of real GDP per capita of Country B and it was growing at 1.4 percent a year, while real GDP...
-
Answer the following questions about break- even analysis: 1. Break- even is defined as the point at which ______________ equals ______________. 2. The contribution margin is equal to ______________...
-
Answer the following questions for: (i) Dyson bladeless fan, (ii) space flight, (iii) cell phone-based GPS.
-
With reference to the Auditors Report, answer the following questions: The recent final audit report of an FMCG carries a line which is read as in the manner so required and respectively give a true...
-
On May 1, 2013, Ramden Company issues 13% serial bonds with a face value of $2 million. The bond contract calls for retirement of the bonds in periodic installments of $200,000, starting on May 1,...
-
In a communication scenario, "people" are best described as _____. A. the audience involved in a communication event and their mental mindsets and contexts that the communicator needs to take into...
-
The Cooper Furniture Company of Potomac, Maryland, assembles two types of chairs (Recliners and Rockers). Separate assembly lines are used for each type of chair. Classify each cost item (AI) as...
-
Lisah, Inc., manufactures golf clubs in three models. For the year, the Big Bart line has a net loss of $10,000 from sales $200,000, variable costs $180,000, and fixed costs $30,000. If the Big Bart...
-
Green Scenery Company provides landscape services and operated based on customer order. The company uses normal costing, and the overhead allocation base is direct labour costs. On 1st May, the...
-
First Trax Company manufactures snowboards. Its standard cost information follows. First Trax has the following actual results for the month of June: Number of units produced and sold...
-
Dell's Working Capital In this case, we will learn about the benefits of effective working capital management by analyzing Dell's financial statements, inventory management, and competitive...
-
You have access to two assets for investing. A Real Estate ETF, which has an expected return of 8 . 1 % , and an Agriculture Commodity ETF which has an expected return of 1 1 . 2 % . You want to...
-
KidzConnect Pte Ltd recently collaborated with a technology company to produce a smart wearable that can be clipped onto a baby's clothing to monitor his/her health. The smart wearable can measure a...
-
23. True or False -- The investment with the lowest equivalent annual cost(charge) will be the least expensive for the firm.
-
Provide an overview of the healthcare economics of the ER, value-based purchasing relevance to patient care, and the financial stability of the er.
-
Graph these combinations of discrete-time functions and continuous-time functions. a) g[n]u[n] + u[n] b) g[n]u[n] - u[-n] c) g(t) = 2u(4- t) d) g(t) = 68(3t+ 9) e) g(t) = 5 ramp(t + 1)
-
What are scruples? Describe a situation where you demonstrated scruples. What are the dangers of social media for law enforcement officers? In what ways do police departments regulate social media...
-
When is the indirect pattern appropriate, and what are the benefits of using it?
-
Multiple Choice Questions 1. Copyhouse Corporation has income before income tax of $160,000 and taxable income of $120,000. The income tax rate is 30%. Copyhouses income statement will report net...
-
Assume that you are a financial analyst. You are trying to compare the financial statements of Caterpillar, Inc., with those of CNH Global, an international company that uses international financial...
-
Crescent Artichoke Growers (CAG) is a major food cooperative. Suppose CAG begins 2010 with cash of $11 million. CAG estimates cash receipts during 2010 will total $104 million. Planned payments will...
-
Name 10 widely used instructional methods.
-
List the five factors that magnify the need for training.
-
What is a training objective? Write a sample training objective in behavioral terms.
Study smarter with the SolutionInn App