Answer

Version B 17. A mutation resulting in a nonfunctional primase enzyme will affect the synthesis of DNA by preventing: A. The DNA double helix from unwinding B. The addition of new nucleic acids onto a growing DNA strand C. The joining of Okazaki fragments D. The initial addition of RNA nucleotides to the template strand. 18. Referring to the figure, what bases will be added as DNA replication proceeds on the bottom strand? 3' HO- G T C T G C T G - OH Template A. C, A, G, C, A, G, A B. T, C, T, G, C, T, G C. A, G, A, C, G, A, C D. G, T, C, G, T, C, T 19. When cells are grown in a medium containing nitrogen 15 ("'N), a stable isotope of nitrogen, it is incorporated into the newly formed DNA. Cells grown in this environment for many rounds of cell division (now containing only 'N in both strands of their DNA) are transferred to a medium that contains only the lighter nitrogen 14 isotope (*N). After one round of cell division: A. Some daughter cells will have only DNA with only 'N, while others will have only 14N B. All daughter cells will have one strand labeled with 'N and the other strand labeled with 14N C. All daughter cells will have DNA that includes a mixture of "N and 14N on both strands D. All daughter cells will only have IN in their DNA 20. Okazaki fragments A. Are present in both the leading and lagging strand during DNA replication B. Are separated by DNA ligase C. Are produced when DNA polymerase III adds nucleotides to the 5' end of a growing strand of DNA D. Are produced when DNA polymerase III works toward the origin of replication during DNA synthesis. Exam continues on next page