Question: Aqueous two - phase extraction is used to purify a recombinant HIV - 3 - galactosidase fusion peptide produced in Escherichia coli. For optimum separation,

Aqueous two-phase extraction is used to purify a recombinant HIV-3-galactosidase fusion peptide produced in Escherichia coli. For optimum separation, 450 kg of a mixture of 19.7% w/w polyethylene glycol (PEG) and 17.7% w/w potassium phosphate salt in water is needed. Left over from previous pilot-plant trials is 100 kg of a mixture of 20% w/w PEG in water, and 150 kg of a mixture of 20% w/w PEG and 25% w/w salt in water. Also on hand is 200 kg of an aqueous stock solution of 50% w/w PEG, 200 kg of an aqueous stock solution of 40% w/w salt, and an unlimited supply of extra water.
If all of both leftover mixtures must be used, how much of each stock solution and additional water is required?

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