Question: Aqueous two - phase extraction is used to purify a recombinant HIV - 3 - galactosidase fusion peptide produced in Escherichia coli. For optimum separation,
Aqueous twophase extraction is used to purify a recombinant HIVgalactosidase fusion peptide produced in Escherichia coli. For optimum separation, kg of a mixture of ww polyethylene glycol PEG and ww potassium phosphate salt in water is needed. Left over from previous pilotplant trials is kg of a mixture of ww PEG in water, and kg of a mixture of ww PEG and ww salt in water. Also on hand is kg of an aqueous stock solution of ww PEG, kg of an aqueous stock solution of ww salt, and an unlimited supply of extra water.
If all of both leftover mixtures must be used, how much of each stock solution and additional water is required?
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