ASSIGNMENT #$8.$

A binding assay was performed with a ligand binding to a receptor. The following binding data were obtained.

$1,940,192,550,268,190,52$

$15,090,7823,160,8268,861,1489,991,15$

Use the one$-$site hyperbolic binding equation to determine Bmax and KD$.$ Show a graph of the data and fitted curve.

Use a software such as the MatLab or R $-$curve fitting tool that uses the method of least squares for curve fitting. Do NOT use methods that linearize data and use a line to approximate constants.

Show $($by for example screenshots$)$ how you did the fitting. Show clearly which tool you used and your procedure.

Free ligand $\backslash$mu M

Bound $\backslash$mu MASSIGNMENT #$8.$

A binding assay was performed with a ligand binding to a receptor. The following binding data were obtained.

$\backslash$table$[[$Free ligand $M,$Bound $M$