Assuming the soil has no appreciable effect on volume, what was the final PNPP concentration in the Soil ALP activity analysis?

**This was the only information given from the experiment**

Please show all your work on how you got the final concentration of PNPP! Thank you :)

thats all the info i got

2.3. Soil ALP activity analysis Soil ALP activity was measured according to the method of Fan et al. (2018). Briefly, 2.0 g soil sample was placed into 20-ml brown glass bottle sand 3 mL Tris-HCl buffer solution with a pH of 8.4 was added. 1 mL 5 mmol L-PNPP (Disodium p-nitrophenyl phosphate) and 1 mL 0.5 mol - CaCl2 were added, respectively. One group (3 replications) of the sample solutions were mixed and placed into a 37 C water bath for 1 h, and another group (3 replications) without water bath (control group). 4 ml 0.5 mol L