(b) presence of oxygen to give o-quinone. The results of reaction rates were as given in...
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(b) presence of oxygen to give o-quinone. The results of reaction rates were as given in experimenti (Table A8.1). In the second experiment, a coupetitive inhibitor was added (parahydroxybenzoe acid (PHBA) and the reaction produced the results of reaction rates as given in experimentz (Table A8.1). In the third experiment, a non-competive inhibitor was added (phenylthioure) the reaction and the results of reaction rates were as given in experiment3 (Table A8.1). (1) Calculate the values of Vmaz and Michaelis Menten constants for each experiment and state wherher they are in conformity witi he concept developed by Lineweaver and Burk. (ii) What is a simple analysis of the results? In an experiment, calechol was reacted with the enzyme diphenol oxidase in the (12 marks) Table A8.1 Experiment results of trials ExperimentI Reaction Rate 0.18 0.12 Experiment2 Substrate Experiment3 Substrate Substrate Reaction Reaction (Sx10) 8,2 (Sx10*) (Sx10) 8.2 rate rate 8.2 0.15 0.11 6.4 6.4 0.09 6.4 0.071 3 4.8 0.083 0.06 0.041 0.026 4.8 4.8 4 1.2 0.048 1.2 0.03 1.2 5 0.7 0.035 0.7 0.02 0.7 0.011 6. 0.3 0.021 0.3 0.01 0.3 0.008 (b) presence of oxygen to give o-quinone. The results of reaction rates were as given in experimenti (Table A8.1). In the second experiment, a coupetitive inhibitor was added (parahydroxybenzoe acid (PHBA) and the reaction produced the results of reaction rates as given in experimentz (Table A8.1). In the third experiment, a non-competive inhibitor was added (phenylthioure) the reaction and the results of reaction rates were as given in experiment3 (Table A8.1). (1) Calculate the values of Vmaz and Michaelis Menten constants for each experiment and state wherher they are in conformity witi he concept developed by Lineweaver and Burk. (ii) What is a simple analysis of the results? In an experiment, calechol was reacted with the enzyme diphenol oxidase in the (12 marks) Table A8.1 Experiment results of trials ExperimentI Reaction Rate 0.18 0.12 Experiment2 Substrate Experiment3 Substrate Substrate Reaction Reaction (Sx10) 8,2 (Sx10*) (Sx10) 8.2 rate rate 8.2 0.15 0.11 6.4 6.4 0.09 6.4 0.071 3 4.8 0.083 0.06 0.041 0.026 4.8 4.8 4 1.2 0.048 1.2 0.03 1.2 5 0.7 0.035 0.7 0.02 0.7 0.011 6. 0.3 0.021 0.3 0.01 0.3 0.008
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From the graph Vmax 01272 in units of rate and KM 0001585 in units of substrate conc Link to ... View the full answer
Related Book For
Fundamentals of Analytical Chemistry
ISBN: 978-0495558286
9th edition
Authors: Douglas A. Skoog,Donald M. West, F. James Holler, Stanley R. Crouch
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