b) The first analyte had a retention time of 7.0 min and a width at the...

 

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b) The first analyte had a retention time of 7.0 min and a width at the base of 0.30 ml. Further experiments revealed that a non-retained compound had a retention time of 1.9 min. The volumetric flow rate was 0.5 ml/min in all experiments. 1) Calculate the retention factor for the first analyte. 2) Calculate the plate number. 3) Calculate the relative retention if the second analyte elutes after 7.5 min. 4) Calculate the peak resolution if the second peak has a width at the base of 0.33 ml. 5) Was a base line peak resolution obtained? If not, what could be done to increase the peak resolution? b) The first analyte had a retention time of 7.0 min and a width at the base of 0.30 ml. Further experiments revealed that a non-retained compound had a retention time of 1.9 min. The volumetric flow rate was 0.5 ml/min in all experiments. 1) Calculate the retention factor for the first analyte. 2) Calculate the plate number. 3) Calculate the relative retention if the second analyte elutes after 7.5 min. 4) Calculate the peak resolution if the second peak has a width at the base of 0.33 ml. 5) Was a base line peak resolution obtained? If not, what could be done to increase the peak resolution?

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1 Retention factor calculation The retention factor k for the first analyte can be calculated using the following equation k tR tM tM where k is the retention factor tR is the retention time of the an... View the full answer