b. Using your new 10M primer solutions, calculate how much of each primer you will need to add when making your PCR Master Mix cocktail to have a final concentration of primers at 0.2M. Show your calculation. (2 points) iii. Hints: iv. What is the total volume of PCR Master Mix you want to make? (see 8a ) v. What is the FINAL concentration of the primers that you want in the PCR Master Mix? (see 8a) vi. What is the INITIAL concentration of the primers? (You just made these primer solutions, look in the question for the concentration) vii. What formula should you use to determine how much of each primer you should add to the other ingredients (buffer, dNTPs, polymerase, water, DNA template) to make the PCR Master Mix