Question: B1. Calculate and enter in Table below how much in volume (ul) of undiluted samples (A to E) and buffer solution (HL) are required to
B1. Calculate and enter in Table below how much in volume (ul) of undiluted samples (A to E) and buffer solution (HL) are required to generate 1 mL (1000 ML) of A1 to El solutions by parallel dilution (1* dilution) with DF1 = 1/50. B2. Continue from the 1st parallel diluted sample, calculate, and enter in Table below how much in volume (ul) of A1 to D1 to generate 0.5 mL (500 ML) of solutions A2 to D2 by make one serial dilution (2nd dilution) with DF2= 1/10. Show your completed table to your GA before proceeding to the next step. To make 500 ML of To make 1000 ml of 2nd serial dilution 1st parallel dilution Sample (DF2 =1/10) Sample Final DF Final (DF, = 1/50), ID from the previous dilution ID (DF: xDF) volume in take _ML takeUL the tube Sample ID (ul) Undiluted sample diluent -1 diluted sample diluent A1 A2 B B1 c C1 B2 C2 D2 500 500 500 500 Keep 500 (Discard the rest) D D1 E E1 1/50
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