B5. Below is the plasmid map of vector pBAD. It has two antibiotic coding genes which...
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B5. Below is the plasmid map of vector pBAD. It has two antibiotic coding genes which gives resistance to ampicillin and chloramphenicol. The respective region of the antibiotic genes is indicated in the plasmid map. The EcoR1 restriction enzyme site is present in chloramphenicol resistance gene indicated by arrow. If I wish to clone a gene at EcoRI site, how would I identify the presence of cloned gene at the enzyme site. (20 Points) UC origis Neel Anc ORF, I'mel EcoRV aRI Aall Chommphenicol e si stance gene PBAD-DEST49 6160 bp Neel met Sma! ced Ampieillin re si stance gene A attR2 all Ma peter B5. Below is the plasmid map of vector pBAD. It has two antibiotic coding genes which gives resistance to ampicillin and chloramphenicol. The respective region of the antibiotic genes is indicated in the plasmid map. The EcoR1 restriction enzyme site is present in chloramphenicol resistance gene indicated by arrow. If I wish to clone a gene at EcoRI site, how would I identify the presence of cloned gene at the enzyme site. (20 Points) UC origis Neel Anc ORF, I'mel EcoRV aRI Aall Chommphenicol e si stance gene PBAD-DEST49 6160 bp Neel met Sma! ced Ampieillin re si stance gene A attR2 all Ma peter
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Related Book For
Organic Chemistry A Short Course
ISBN: 978-1111425562
13th edition
Authors: Harold Hart, Christopher M. Hadad, Leslie E. Craine, David J. Hart
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