Question: Biochem lab question 5) (4 pts) If you make up 100 mL of 0.2 M TRIS in the acid form, what would the pH be?
Biochem lab question
5) (4 pts) If you make up 100 mL of 0.2 M TRIS in the acid form, what would the pH be? (if desired, you may assume that the amount of protons that dissociate from the TRIS are negligible relative to the total TRIS concentration) 6) (4 pts) Next, you add 3 mL of 1 M NaOH to the solution in the problem above. What is the pH of the solution after the addition of NaOH? Note that for this question, you can assume that since you are using the acid form of TRIS, 100% of the TRIS species will be in the acid (HA) form prior to the addition of the NaOH
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