6. Using the reference gel provided (do NOT use your own gel to answer the following...

 

Transcribed Image Text:

6. Using the reference gel provided (do NOT use your own gel to answer the following questions): a. Label each band on the gel with its approximate size. (4) b. Which plasmid was isolated from Culture A? Use the results from the gel, as well as the predicted results (plasmid maps are included in this assignment, please use these maps, not the ones in your lab manual) to support your answer. (2) c. Which plasmid was isolated from Culture B? Use the results from the reference gel, as well as the predicted results (plasmid maps are included in this assignment, please use these maps, not the ones in your lab manual) to support your answer. (2) d. Was the plasmid from Culture A relaxed? Supercoiled? Or both? Explain using the results from the gel. (2) e. Was the plasmid from Culture B relaxed? Supercoiled? Or both? Explain using the results from the gel. (2) Biology 107 Lab Assignment 4: Plasmid ID Klobases 50 20- 15- 12- 1.0- 3000bp 07- 06- 1000bp 04- 03- 500bp 02- 01- Reference gel to use for question 6 Molecular Weight Marker used for gels AmpR Hind III (1234) PHSG (2673 bp) (3337 bp) Hind III (234) Hind II (1734) Plasmid maps for pGFPuv and pHSG Hindii Culture A Uncut Culture A Hindil Culture Uncut Culture KanR 6. Using the reference gel provided (do NOT use your own gel to answer the following questions): a. Label each band on the gel with its approximate size. (4) b. Which plasmid was isolated from Culture A? Use the results from the gel, as well as the predicted results (plasmid maps are included in this assignment, please use these maps, not the ones in your lab manual) to support your answer. (2) c. Which plasmid was isolated from Culture B? Use the results from the reference gel, as well as the predicted results (plasmid maps are included in this assignment, please use these maps, not the ones in your lab manual) to support your answer. (2) d. Was the plasmid from Culture A relaxed? Supercoiled? Or both? Explain using the results from the gel. (2) e. Was the plasmid from Culture B relaxed? Supercoiled? Or both? Explain using the results from the gel. (2) Biology 107 Lab Assignment 4: Plasmid ID Klobases 50 20- 15- 12- 1.0- 3000bp 07- 06- 1000bp 04- 03- 500bp 02- 01- Reference gel to use for question 6 Molecular Weight Marker used for gels AmpR Hind III (1234) PHSG (2673 bp) (3337 bp) Hind III (234) Hind II (1734) Plasmid maps for pGFPuv and pHSG Hindii Culture A Uncut Culture A Hindil Culture Uncut Culture KanR

Answer rating: 100% (QA)

A b The size f the unut ulture bnd in the gel is ner 3000 b But the HindII ut ulture shws nly ne bnd ... View the full answer