Can someone help me with writing protocols to test two hypothesis? Thank you very much!

Here is the question:

You have successfully identified a gene required for cell division in yeast called Cdc2. Yeast with loss-of-function mutations in Cdc2 cannot divide properly. You are particularly interested in theproteinencoded by Cdc2 because your data suggests that the causative mutation does not affect gene expression. In other words, you observed no difference in the relative abundance of RNA transcript in the mutant.

You will write two DIFFERENT protocols (descriptions of methods) that would allow a researcher to test the hypotheses outlined below. You can and should use any and all resources available to you (computers, textbooks, classmates, instructors, etc). Be specific! A well-written protocol should allow you and your colleagues to perform an experiment successfully without having to refer to other sources. Finally,you must use a different technique/approach, or set of techniques and approaches in each protocol.

Hypothesis 1: The sub-cellular localization of the protein is altered in the mutant and thus it will be located in a different part of a mutant cell relative to that of a wild-type cell.

Hypothesis 2: The protein is targeted for degradation in the mutant and thus is less abundant or even absent in mutant yeast.

The following is a list of things that could appear in at least one of your protocols.

• antibodies that recognize and bind to your protein (made in a rabbit) and coupled or uncoupled to a fluorescent dye
• a-rabbit antibodies with fluorophores
• light or electron microscopes in the lab
• a sonicator
• a centrifuge (note that proteins remain in the supernatant during high-speed centrifugation)
• SDS and mercapoethanol
• Polyacrylamide gel
• Pipettes
• Nitrocellulose paper
• Total protein
• Yeast cells
• Plates with growth media
• An incubator

Most importantly, you SHOULD BE ABLE TO explain WHY each step makes sense and is significant.