can someone please help me with the 7 post lab analysis , I wasnt able to attend lab .

1. Using an excel to find the average absorbance value for blank tube and tubes (1-7)
2. Subtract the average absorbance value of the blank tube from tubes 1-7 to make

3. Plot Absorbance (Y axis) vs. concentration (X axis) of tubes 1-7 (standard solution) after

absorbance correction

4. Select the equation of y = mxt b between the relationship between absorbance and concentration of BSA. y represents absorbance; x represents concentration (amount) of BSA; m is the slope

5. Find x from y = mx + b where x = (y-b)/

6-Calculate the concentration of sample A and B from measured absorbance. Substitute Measured absorbance of samples A or B as "y" in the equation (y-b)/m to calculate X; b and m values are given by the equation on the excel

7 Calculate the original concentration of protein in skim milk

Lab: Modified Bthdford Assay 1252023 Prosecol: 1. 20 (1.Smi) centrifuge tubes and lubel accotding so the table below. See colunn I and 2 2. Add waltr and BSA (seck) to each cabe at shoan below. Tube blank to mbe 7 3. Dilute 1.90 of skim milk with watce in a veal of s000l. Add 50al of dilured skim mik in: twere A and B below. 4. Aad 1mL of dye ragent we each tube. When the magent a added cumpletely. Clone the caps 5. Mat restion tubes gendly by ineting 34 time to mure the whotive in mixad midonmy. 6. Ineubute the racton rubsi for 30 minuass at poem tenveratare abuchance at ses nat Fost AB ANALYsIS warroctint. Wisoabance conroctian: issh: wi ibe dupe 5. Find x trem y=x+b bter x(yb)= Lab: Modified Bthdford Assay 1252023 Prosecol: 1. 20 (1.Smi) centrifuge tubes and lubel accotding so the table below. See colunn I and 2 2. Add waltr and BSA (seck) to each cabe at shoan below. Tube blank to mbe 7 3. Dilute 1.90 of skim milk with watce in a veal of s000l. Add 50al of dilured skim mik in: twere A and B below. 4. Aad 1mL of dye ragent we each tube. When the magent a added cumpletely. Clone the caps 5. Mat restion tubes gendly by ineting 34 time to mure the whotive in mixad midonmy. 6. Ineubute the racton rubsi for 30 minuass at poem tenveratare abuchance at ses nat Fost AB ANALYsIS warroctint. Wisoabance conroctian: issh: wi ibe dupe 5. Find x trem y=x+b bter x(yb)=