can you please draw the map for me and upload as pdf $1.$ Start with a circle:

Draw a circle to represent the plasmid.

$2.$ Divide the circle into proportional segments:

Draw five sections that are proportional in size to their lengths:

$4,000$ bp

$3,000$ bp

$1,650$ bp

$1,000$ bp

$500$ bp

$3.$ Label the fragments:

Write the size of each fragment in its box.

$4.$ Mark TN$5$ insertion sites:

Place an arrow into each fragment to indicate into which fragment TN$5$ had been inserted:

For the $4,000$ bp fragment: Mark this as a Lane $2$ insertion.

For the $3,000$ bp fragment: Mark this as a Lane $3$ insertion.

For the $1,650$ bp fragment: Mark this as a Lane $4$ insertion.

For the $1,000$ bp fragment: This is the insertion site for Lane $5.$

For the $500$ bp fragment: This is the insertion site for Lane $6.$

Step $5$: Add a legend

Create a legend to describe the map:

Arrows represent TN$5$ insertion sites.

Sizes are original restriction fragments.

Example $($in words$)$:

Imagine a clock face:

The $12-3$ o'clock position is $4,000$ bp$.$ This is the TN$5$ insertion from Lane $2.$

The $3-6$ o'clock region is the $3,000$ bp TN$5$ insertion from Lane $3.$

The $6-7$:$30$ o'clock region is the $1,650$ bp TN$5$ insertion from Lane $4.$

The $7$:$30-9$:$30$ o'clock region is the $1,000$ bp TN$5$ insertion from Lane $5.$

The $9$:$30-12$ o'clock region is the $500$ bp TN$5$ insertion from Lane $6.$