Categories into function, expression, or both

You are interested in analyzing a new protein that you have discovered in mice, which is encoded by the YFG gene. You would like to understand both the function and expression pattern of the protein. Sort the following techniques into those that can provide information about function, and those that can provide information about expression. Items (5 items) (Drag and drop into the appropriate area below) Generate a YFG Generate an RNA Make a "knock- Construct a Use RNAi to knockout mouse. probe for YFG in" mouse where YFG-GFP (green knock down YFG and perform in both copies of fluorescent mRNA levels. situ the YFG coding protein) fusion hybridization. sequence are protein and use it replaced with to make a GFP (green transgenic fluorescent mouse. protein)