Cefixime is an antibiotic that works by blocking synthesis of cell walls in bacteria. It is...
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Cefixime is an antibiotic that works by blocking synthesis of cell walls in bacteria. It is hypothesized that serum albumin binds to this drug to deliver it throughout the body. In Cheng L-, Yang C-, Li H-, Li M, Bai A-, Ouyang Y, Hu Y-. Probing the interaction of cephalosporin with bovine serum albumin: A structural and comparative perspective. Lumin 2018;33(1):209-18, the authors studied the interaction of Cefixime to serum albumin as a function of temperature. Some of their results are below: 10-r/Df 8.0 7.2 6.4 5.6 4.8 4.0 3.2 (b) 0.15 0.30 0.45 r T = 292 K O T = 298 K T = 304 K * T = 310 K 0.60 0.75 Note that the authors do not show the x intercept in this plot, and all curves reach approximately a value of 2 at the x intercept. a) Is there evidence for cooperativity in binding at 310K, and if so, is it positive or negative? (Minimally describe the differences in the expected Scatchard plots if there is positive, negative or no cooperativity. )If you aren't sure, would it be better to get more data at a lower ligand concentration or a higher ligand concentration (why?). Cefixime is an antibiotic that works by blocking synthesis of cell walls in bacteria. It is hypothesized that serum albumin binds to this drug to deliver it throughout the body. In Cheng L-, Yang C-, Li H-, Li M, Bai A-, Ouyang Y, Hu Y-. Probing the interaction of cephalosporin with bovine serum albumin: A structural and comparative perspective. Lumin 2018;33(1):209-18, the authors studied the interaction of Cefixime to serum albumin as a function of temperature. Some of their results are below: 10-r/Df 8.0 7.2 6.4 5.6 4.8 4.0 3.2 (b) 0.15 0.30 0.45 r T = 292 K O T = 298 K T = 304 K * T = 310 K 0.60 0.75 Note that the authors do not show the x intercept in this plot, and all curves reach approximately a value of 2 at the x intercept. a) Is there evidence for cooperativity in binding at 310K, and if so, is it positive or negative? (Minimally describe the differences in the expected Scatchard plots if there is positive, negative or no cooperativity. )If you aren't sure, would it be better to get more data at a lower ligand concentration or a higher ligand concentration (why?).
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Related Book For
Organic Chemistry A Short Course
ISBN: 978-1111425562
13th edition
Authors: Harold Hart, Christopher M. Hadad, Leslie E. Craine, David J. Hart
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