Chapter 5 Worksheet 1. For the following procedures indicate how they separate proteins (or amino acids): a. Electrophoresis: b. Size Exclusion Chromatography: c. Affinity Chromatography: d. Ion Exchange Chromatography: e. Isoelectric focusing: 2. If the following proteins were ran on a size exclusion chromatography column, which would come off the column last? 3. As you run experiments to isolate a protein, what would you expect to happen to the. a. Percent recovery: b. Purification (Specific Activity): 4. Predict where the following amino acids would fall on a gel when running an isoelectric focusing experiment. Which would migrate the furthest on the gel? 5. The following amino acids were ran on a cation exchange chromatography column at a pH of 8.5; cysteine, arginine, and tryptophan. Determine which amino acid would come off last