CHEN 4311.001 HOMEWORK 2 SPRING 2022 NAME CHAPTER 3 DUE DATE: 02/14/2022 Introduction to Enzymes (10 points total) 1. Derivation of a rate expression for two consecutive reactions (10 points): Consider the following reaction sequence: ky S+E [ES), [ES ky ki APIE k2 Develop a suitable rate expression for the product P formation Irks (ES121 by using: a. the quasi-equilibrium (Michaelis-Menten) approach b. the pseudo-steady-state (Briggs-Haldane) approach Show all of your algebraic steps for both methods. 2. Simple enzymatic reaction calculations (10 points): The enzyme fumarase has the following kinetic constants: ky S+E [] (ES) .P+E kus Where kg = 10M-1,- k144 1093 kz= 101 a. What is the value of the Michaelis-Menten constant for this enzyme? b. At an enzyme concentration of 10M, what will be the initial rate of product formation at a substrate concentration of 10-M