Question: Chromatograms were obtained for two different compounds A and B by injecting pure samples of these substances into a 30-cm long column. In both experiments,

 Chromatograms were obtained for two different compounds A and B by

Chromatograms were obtained for two different compounds A and B by injecting pure samples of these substances into a 30-cm long column. In both experiments, the same mobile phase flow rate was used. We would like to separate A and B at the same mobile phase flow rate from a mixture containing the same amounts of these substances as used for obtaining the chromatograms. The mobile phase residence time is 2 minutes and the voidage fraction is 0.3. Calculate the theoretical plate height of the chromatographic column, the selectivity, and the resolution. If we collect the column effluent from the start to 7 minutes, calculate the purity of A in the sample and the percent yield

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