Could you please help me to answer the calculation questions, thank you !

Phenylbutazone-BSA binding assay. 1. Each 500 uL binding assay reaction mix comprises phenylbutazone, with or without BSA, in PBS solution. 2. [PB]o and [BSAJo are the final concentrations of phenylbutazone and BSA in the reaction mix, respectively. Prepare the reaction mixes as indicated in Table 2. Table-2 [PB). [BSA). Volume of Weight of Volume of Absorbance PB stock BSA (mg) PBSA (UL) (2 readings (UL) avg) Sample 50.00 55.00 62.5 1.828 437.5 10.0160.012 Control-1 50.00 0 62.5 437.5 0.0126.00$ 0 Control-2 0 0 500 4 o Calculations 1. Use the phenylbutazone absorbance calibration curve to calculate the concentrations (C) from the absorbance data in Table 2. 2. Estimate the fraction of PB that is bound to BSA. 3. Calculate Ko and AGo, assuming the temperature to be 22C and n=1. Phenylbutazone-BSA binding assay. 1. Each 500 uL binding assay reaction mix comprises phenylbutazone, with or without BSA, in PBS solution. 2. [PB]o and [BSAJo are the final concentrations of phenylbutazone and BSA in the reaction mix, respectively. Prepare the reaction mixes as indicated in Table 2. Table-2 [PB). [BSA). Volume of Weight of Volume of Absorbance PB stock BSA (mg) PBSA (UL) (2 readings (UL) avg) Sample 50.00 55.00 62.5 1.828 437.5 10.0160.012 Control-1 50.00 0 62.5 437.5 0.0126.00$ 0 Control-2 0 0 500 4 o Calculations 1. Use the phenylbutazone absorbance calibration curve to calculate the concentrations (C) from the absorbance data in Table 2. 2. Estimate the fraction of PB that is bound to BSA. 3. Calculate Ko and AGo, assuming the temperature to be 22C and n=1