create a serial dilution procedure table using 11 concentration standards for the test tubes in the range of 5-2000 ug/mL and 1 for the blank.

Materials 600-mL beaker Scoopula Coomassie Brilliant Blue G-250 DI wash bottle 95% ethanol Glass stir rod 85% phosphoric acid 25-mL volumetric pipet 50-mL volumetric pipet 50- mL graduated cylinder 500-mL volumetric flask 60 test tubes (15100mm) Procedure I. Preparation of Bradford Reagent 1. Weigh 0.050g of solid Coomassie Brilliant Blue G-250 and quantitatively transfer to a 600mL beaker. 2. Dissolve the solid in 25mL of 95%EtOH. 3. In the fume hood, add 50mL of 85% phosphoric acid. 4. Add 50mL of DI water. 5. Stir until all dye is dissolved. 6. Filter the dye solution to remove any precipitant that may form. 7. Qualitatively transfer the solution to a 500 -mL volumetric flask and further dilute to the mark with 1. Make up a set of standards using BSA or some other protein source in the range of 52000g/mL. In the Bradford procedure, you will use 0.1mL of each sample. 2. To 0.1mL of your unknown samples (and dilutions of your samples), and to 0.1mL of the standards add 3mL of Bradford reagent. Mix well and let stand at room temperature for 5min. The unknowns and the standards should be measured in the same experiment. 3. Measure the absorbance at 595nm against a blank consisting of 100L of sample buffer or water and 3mL of the dye reagent. 4. Determine your protein concentration(s) from a standard curve