Question: d. The chromatogram below was the first attempt at separating this four-component mixture. This initial separation was carried out using a mobile phase of (

d. The chromatogram below was the first attempt at separating this four-component mixture. This initial separation was carried out using a mobile phase of ( 50 % ) methanol/50% water. What was changed about the method to achieve the chromatogram shown above (full separation)? Why did this change lead to an improved separation? (6 pts)

d. The chromatogram below was the first attempt at separating this four-component mixture. This initial separation was carried out using a mobile phase of 50% methanol /50% water. What was changed about the method to achieve the chromatogram shown above (full separation)? Why did this change lead to an improved separation? ( 6 pts)

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