Develop a

purification train

for a facility where

first process is a

perfusion upstream

bioreactors $500$L

producing low cell

culture titer of

approx. $0\mathrm{.}5$g$/$L

perfusing at $2$ VVD

over $30$ daysThe current facility has a secondary clarification process for the

perfusate coming from the bioreactor. Secondary depth filtration

clarification capacity of $200$ L$/$m$2.$ Identify the correct filter area, and

system $($pump$)$ requirements for the process scale. Also identify

optimal flowrates for flushing and processing, total process time,

buffer volumes required. Assume $10$ L$/$m$2$ holdup of the depth filters

identify the size of the tank required to collect the filtrate. Average

yield of overall clarification is $80\%$ estimate the titer in the clarified

pool