Drag and drop the appropriate text in the gaps below. Firstly, the plasmid DNA in the E. coli is propagated through . Then the bacterial cells are pelleted by centrifugation at 10,000 RPM. The media supernatant is removed and the bacterial cells are . The bacterial cells are then lysed via . Contaminating macromolecules such as protein and chromosomal DNA is precipitated by . Overnight incubation of the bacterial culture washed in cell resuspension buffer addition of lysis buffer addition of neutralisation buffer washing of the spin column separation via agarose gel electrophoresis