Question: During a fed - batch bioreactor run 1 M NaOH ( ~ 1 6 0 0 mM ) is used to control the pH .

During a fed-batch bioreactor run 1M NaOH (~1600 mM) is used to control the
pH. In addition, a feed solution with an osmolarity of 1500 mOsm is used for fed-
batch operation. The starting cell culture medium has an osmolarity of 320
mOsm. If the total base addition was 30 mL/L of culture and if the total feed was
300 mL/L of the culture, estimate the final osmolarity.

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