Exercise I An HPLC method was developed for the separation and determination of ibuprofen in rat plasma samples as part of a study of the time course of the drug in laboratory animals. Several standards were chromatographed and the results below obtained Ibuprofen concentration (g/mL.) Peak Arca 0.5 5.0 1.0 10.1 20 172 3.0 19.8 6.0 39.7 80 573 10,0 66,9 15.0 93.3 Next, a 10-mg/kg sample of ibuprofen was administered orally to a laboratory rat. Blood samples were drawn at various times after administration of the drug and subjected to HPLC analysis. The following results were obtained Time, hr. Peak Area 0 0 05 91.3 10 80.2 1.5 $2.1 2.0 38.5 3.0 24.2 4.0 21.2 18.5 8.0 15.2 1. Find the concentration of ibuprofen in the blood plasma for each of the times given above and plot the concentration versus time 2. On a percentage basis, during what half-hour period (Ist, 2nd, 3rd, ...) is most of the ibuprofen lost