Question: For HEPES buffer is pH 4.28, THE pKa is 7.55 a. What is the ratio of A-/ HA in the buffer that is present in

For HEPES buffer is pH 4.28, THE pKa is 7.55

a. What is the ratio of A-/ HA in the buffer that is present in the solution?

b. How many micromoles of A- and HA are present in the solution?

c. If you now add 3 ml of 1M NaOH, will you still have a valid buffer? Explain.

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