General Instructions: Use Excel (or your favorite graphing program) to make the data plots and include the graphs in your submission. For all questions requiring calculations, show your work, include 2 significant digits after the decimal and include units for full credit. Place a box around your final answer. Include a title and axes on all plots. 1. Experimental data of a multi-subunit protein binding its ligand is shown below. a) (2 points) Construct a Hill plot of the binding data using Excel. b) (1 point) What is the maximum amount of cooperativity between the subunits? (Hint: select the four adjacent data points that give the steepest slope in the hill plot) c) (1 point) What is the minimum number of ligand binding sites on the protein? 2. The following initial velocity data were measured in an enzyme catalyzed reaction as a function of substrate concentration in the absence and presence of an inhibitor