Hand written solution required

Advanced Physics We follow the kinetics of hydrolysis of orthonitrophenyl-B-D-galactopyranosie (ONPG) by B-galactosidase, respectively in the absence of inhibitor (A) and in the presence of orthonitrophenyl- BD thiogalactopyranosie (ONPTG) (B) , maltose (a- D-glucopyranosyl-(1,4)-B-D-glucopyranose) (C) or melibiose (a-D-galactopyranosyl (1,6)-a-D- glucopyranose)(D). The values of the initial velocities obtained by following the variation of the absorbance at 1 = 410 nm are as follows: Vitesse de la reaction ONPG (UM AcetylCoA hydrolysee/min/mg de proteine) (UM) A B C D 25 0,033 0,018 0,016 0,027 50 0,055 0,033 0,027 0,041 100 0,082 0,055 0,041 0,055 250 0, 118 0,091 0,059 0,069 500 0,138 0, 118 0,069 0,075 1000 0,150 0,138 0,075 0,079 Determine VM, KM and kcat (in s-1) using the representation of your choice. - Determine the kinetic parameters VMxapp and KMapp in the presence of inhibitors. - Calculate the KI constants. - Explain the type of inhibition observed for each of the inhibitors of this exercise. Data: [Eo] = 1.19 10-9 M - EMONP = 3300 M-1.cm-1 - | = 1 cm