Question: he immobilized enzyme layer shown in the figurebelow is designed to convert substrate A to product B accordingto the simplified reaction A B with simplified

he immobilized enzyme layer shown in the figurebelow is designed to convert substrate A to product B accordingto the simplified reaction A B with simplified rate equationRA k cA. In this process, the steady-state concentrations ofsubstrate A in the bulk liquid and the interface between theenzyme layer and its inert support surface were measured, withcA 1.0 mmol/m3 at z 0, and cAL 0.25 mmol/m3 at z L.The effective diffusion coefficient of substrate A within theenzyme layer is DAe 2.0105 cm2/s, and the thicknessof the immobilized enzyme layer is L 0.2 cm. The surroundingfluid is well mixed, and the concentration of substrate A at thesurface of the enzyme layer can be taken as the bulk concentra-tion of substrate A in the surrounding liquid.

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