Question: he immobilized enzyme layer shown in the figurebelow is designed to convert substrate A to product B accordingto the simplified reaction A B with simplified
he immobilized enzyme layer shown in the figurebelow is designed to convert substrate A to product B accordingto the simplified reaction A B with simplified rate equationRA k cA In this process, the steadystate concentrations ofsubstrate A in the bulk liquid and the interface between theenzyme layer and its inert support surface were measured, withcA mmolm at z and cAL mmolm at z LThe effective diffusion coefficient of substrate A within theenzyme layer is DAe cms and the thicknessof the immobilized enzyme layer is L cm The surroundingfluid is well mixed, and the concentration of substrate A at thesurface of the enzyme layer can be taken as the bulk concentration of substrate A in the surrounding liquid.
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