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5 Multiple Choice 1 point When [protein]=[complex] how much of the total protein (ignore ligand) is in the complex? Kd = [protein][ligand]/[complex] O all O half O can't figure this out 6 Multiple Choice 1 point What would be true of a protein ligand interaction with very high affinity? O Ka would vary depending on concentration of the protein Ky would be high O Ky would be low 7 Multiple Choice 1 point It's thought that avidin acts as an antimicrobial as biotin (vitamin B7) is necessary for many bacteria. How much stronger is the interaction between Avidin and B7 than the interaction between insulin and the insulin receptor? Protein-ligand dissociation constants (K 's) vary over several orders of magnitude TABLE 5-1 Some Protein Dissociation Constants Protein Ligand Avidin (egg white) Biotin 1 X 10-15 Insulin receptor (human) Insulin 1 x 10-10 Anti-HIV immunoglobulin (human)* gp41 (HIV-1 surface protein) 4 X 10-10 Nickel-binding protein (E. coli) Ni2+ 1 X 10-7 Calmodulin (rat)5 Ca2+ 3 X 10- 2 X 10-5 Typical receptor-ligand interactions Sequence-specific protein-DNA Biotin-avidin Antibody-antigen Enzyme-substrate 10 16 10-14 10 12 10-10 10 8 10 6 10-4 high affinity Ka (M) low affinity Table 5-1 theinger Principles of Blochemistry Fifth Edition 2008 W.H.Freeman and Company