Hey,

I need help with the following question from pharmaceutical analytical chemistry!

Thank you!

Describe how you would practically carry out the analysis below, including which experimental conditions you choose (remember to justify why you choose these particular conditions). You do not need to describe how to validate your method or perform any calculations in this task. A content determination using the external standard method of a drug candidate which is a weak base with known identity and the pka value 10.7. as a separation method you should use liquid-liquid extraction (LLE) and at the start of the experiment you have the base in the aqueous phase, and want to transfer the base to the organ phase (keep in mind that the pH in water is about 7). Then use the external standard method with mass spectrometry (MS) as the detection method for determining the content of the base. Assume that you know within which concentration range your substance should be present and know all the necessary physical chemistry data. Pay special attention to the choice of solvent for solvation of the analyte in the liquid-liquid extraction, as well as to explain the process of quantification with an external standard. Also remember to explain which ionization technique you choose, and justify your choice. Assume that redn reference substance is available for the analyte