Question: HIGH PERFORMANCE CHROMATOGRAPHY (HPLC) Question 1: Substance A and substance B have very similar absorption spectra, with peaks at 350nm and 352nm. How does this

HIGH PERFORMANCE CHROMATOGRAPHY (HPLC)

Question 1: Substance A and substance B have very similar absorption spectra, with peaks at 350nm and 352nm. How does this influence the chromatogram of these 2 substances?

Question 2: What is the influence of the length of the column on the separation of the peaks in the chromatogram?

Question 3: Suppose that there is an additional compound in the mixture and that it is capable of carrying out the absorption process. However, it is not observed in the chromatogram. Explain the possible reasons why the peak is not observed.

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