Question: HIGH PERFORMANCE CHROMATOGRAPHY (HPLC) Question 2: At what wavelength is the highest absorbance of each component of the mixture obtained? Question 3: What is the

HIGH PERFORMANCE CHROMATOGRAPHY (HPLC)

Question 2: At what wavelength is the highest absorbance of each component of the mixture obtained? Question 3: What is the influence of the particle diameter of the stationary phase. Explain what is the reason for the greater or lesser separation of the peaks. Question 4: Explain the influence of the polarity of the mobile phase to achieve a better separation of the peaks. When do I get a better separation, at low polarity or at high polarity? Question 5: Suppose that there is an additional compound in the mixture and that it is capable of carrying out the absorption process. However, it is not observed in the chromatogram. Explain the possible reasons why the peak is not observed.

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