Question: How does DNA impurity cause incomplete digestion in restriction enzyme reactions? My insert has an A260/A280 ratio of 1.72 and my vector has a A260/A280

How does DNA impurity cause incomplete digestion in restriction enzyme reactions? My insert has an A260/A280 ratio of 1.72 and my vector has a A260/A280 ratio of 2.20. The ideal range is 1.8-2

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