How does the polypeptide translated from the tra-RB isoform differ from the polypeptide translated from the tra-RA isoform? What are the consequences of these differences on protein function? Discuss how the bigger mRNA leads to creation of a smaller polypeptide!! Consider how alternative splicing could allow many different proteins to be encoded by the same gene. Based on the gene structure of the two isoforms of tra shown in the "FlyBase Genes" track, provide a hypothesis that could explain this difference in RNA-Seq read coverage between the adult males sample and adult females sample. Ch (United States) Focus 9999 MacBook Air