how to make a 25x stock solution if extraction buffer with 1 cOmplete tablet? Note:if we want 250 mL final amount of the solution (more info below)

Preparation Note

Working concentration: 1 tablet per 50 ml extraction solution Working solution: Preparation of Working Solution: One cOmplete Protease Inhibitor Tablet is sufficient for the inhibition of the proteolytic activity in 50 ml extraction solution. If very high proteolytic activity is present, one tablet should be used for 25 ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively, a stock solution (25 X conc.) can be prepared. Preparation of Stock solution (25 X conc.): Dissolve one cOmplete Protease Inhibitor tablet in 2 ml dist. water or in 2 ml 100 mM phosphate buffer, pH 7.0. The stock solution can also be prepared in 10 mM Tris buffer, pH 7.2 to 7.5. Storage conditions (working solution): The stock solution can be stored at 2 to 8 C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 C.

Reconstitution

Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 25x stock solutions in 2 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1 to 2 weeks at 2 to 8 C or at least 12 weeks at -15 to -25 C; can also be used in thiol-containing solutions at 15 to 25 C.