How would you prepare 1.0 L of a 150 mM Tris buffer at pH 7.8 using Tris

Fantastic news! We've Found the answer you've been seeking!

Question:

How would you prepare 1.0 L of a 150 mM Tris buffer at pH 7.8 using Tris base crystalline (MW of Tris base is 121g/mol) and a solution of 0.2 M HCl (pKa for Tris acid is 8.08)?

Related Book For answer-question

Fundamentals of Analytical Chemistry

ISBN: 978-0495558286

9th edition

Authors: Douglas A. Skoog,Donald M. West, F. James Holler, Stanley R. Crouch

See More Books

Posted Date: Oct 02, 2020 05:53 PM

See More Questions

You have 1 liter of 0.1M TRIS buffer at pH = 8. The pKa for TRIS is 8.08. How much 10M HCl would you need to make this buffer pH at 7.0? Do not concern yourself with dilution effects. a. 2 ml b. 4 ml...
How would you prepare 1.00 L of a buffer with a pH of 7.00 from 0.200 M H3PO4 and 0.160 M NaOH?
How would you prepare the following compounds from the given starting materials? a. b. c. d. CH3CH2CH CH3CHCH N(CH3)2 CH CHCH OCH3
Research is a process of discovering new knowledge. In the Code of Federal Regulations (45 CFR 46.102(d)) pertaining to the protection ofhuman subjects,research is defined as:...
A random sample of 748 voters in a large city were asked how they voted in the presidential election of 2008. Calculate chi square and the column percentages for each table below and then write a...
A prey population with a curved zero growth isocline is important because the zero growth isocline of the predator might intersect it to either the right or the left of the maximum value. One...
1. Find the z-score that corresponds to a cumulative area of 0.3632. 2. Find the z-score that has 10.75% of the distributions area to its right.
Differential Analysis for Sales Promotion Proposal Sole Mates Inc. is planning a one-month campaign for May to promote sales of one of its two shoe products. A total of $130,000 has been budgeted for...
1. What is organization theories in action? 2. What is nokia organization theory in action?
A solar collector design consists of several parallel tubes each enclosed concentrically in an outer tube which is transparent to solar radiation. The tubes are thin walled with diameter of the inner...
If the sum of lengths of the hypotenuse and a side of a right angled triangle is given, show that the area of the triangle is maximum, when the angle between them is ? / 3.
A company sells one model of a synthetic leather basketball. The basketballs sell for $20 each, and the variable expenses for each basketball are $14. The company has $1,200 of fixed expenses. How...
If a $2000, one year 7% bond is sold for $2841, what is included in the journal entry to record the bond issuance? Show your calculations.
The corporation has 150,000 shares of $2 par common stock authorized and 52,000 shares are currently outstanding. Treasury stock has debit of $26,700. What is the number of shares issued?
Solve the equation. Write the answer in terms o e8x+3=14 The solution set is (Type an exact answer in simplified form. Use in separate answers as needed.)
A lab building had completed an addition, in the year of 2019, that cost $3,000,000 when the relevant cost index was 206. Today, when the latest cost index is 269, they wish to build a similar...
Table 1.2 2020 Federal Tax Rates for Individual and Joint Taxpayers Income brackets Income Tax Rate Individual Taxpayers Joint Taxpayers 10% $0 to $9,875 12% $9,876 to $40,125 $0 to $19,750 $19,751...
Explain the buyers position in a typical negotiation for a business. Explain the sellers position. What tips would you offer a buyer about to begin negotiating the purchase of a business?
Three different analytical methods are compared for determining Ca in a biological sample. The laboratory is interested in knowing whether the methods differ. The results shown below represent Ca...
A 0.917-g sample of canned tuna was analyzed by the Kjeldahl method. A volume of 20.59 mL of 0.1249 M HCl was required to titrate the liberated ammonia. Calculate the percentage of nitrogen in the...
What experimental variables must be controlled to assure reproducible absorbance data?
How are the identifiable assets and liabilities acquired in a business combination measured?
What is meant by deferred consideration and how is it accounted for?
Distinguish between a business combination and a non-business acquisition.

How would you prepare 1.0 L of a 150 mM Tris buffer at pH 7.8 using Tris

Question:

Expert Answer:

Fundamentals of Analytical Chemistry

Students also viewed these chemistry questions